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I Vivo Målretning af neurale progenitor celler i Ferret Neocortex ved In Utero Electroporation
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Neurociência
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JoVE Journal Neurociência
In Vivo Targeting of Neural Progenitor Cells in Ferret Neocortex by In Utero Electroporation

I Vivo Målretning af neurale progenitor celler i Ferret Neocortex ved In Utero Electroporation

DOI:
10.3791/61171-v

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07:03 min

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May 06, 2020

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, , , ,
1Max Planck Institute of Molecular Cell Biology and Genetics, 2Human Technopole, 3Landesdirektion Sachsen, 4Department of Medical Neuroscience, Graduate School of Medical Sciences,Kanazawa University

Capítulos

  • 00:04Introduction
  • 00:43In Utero Electroporation and Surgical Preparation
  • 02:13In Utero Electroporation
  • 04:45Results: Representative Ferret Neocortex Imaging
  • 06:13Conclusion

Summary

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Tadução automática

Præsenteret her er en protokol til at udføre genetisk manipulation i embryonale ilder hjernen ved hjælp af i livmoderen elektroporation. Denne metode giver mulighed for målretning af neurale progenitor celler i neocortex in vivo.

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