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In Vivo Targeting of Xenografted Human Cancer Cells with Functionalized Fluorescent Silica Nanoparticles in Zebrafish
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1Departments of Pharmacology and Medicine, Section of Hematology and Medical Oncology, The Cancer Research Center,Boston University School of Medicine, 2Department of Biomedical Engineering,Tufts University, 3Department of Mechanical Engineering,Tufts University, 4Department of Physics,Tufts University
Capítulos
- 00:04Introduction
- 00:54Generation of Casper Zebrafish Embryos
- 02:33Preparing Materials for Cancer Cell Transplantation
- 03:34Preparing Cancer Cells for Transplantation
- 05:20Transplantation of Cancer Cells
- 06:54Injection of Nanoparticles or Vehicle
- 07:40Imaging and Tracking of Nanoparticles and Cancer Cells
- 08:45Results: Colocalization of Cancer Cells and Nanoparticles
- 09:47Conclusion
Hier beschreven is een methode voor het gebruik van zebravis embryo's om het vermogen van gefunctionaliseerde nanodeeltjes te bestuderen om menselijke kankercellen in vivo richten. Deze methode maakt de evaluatie en selectie van optimale nanodeeltjes mogelijk voor toekomstige tests bij grote dieren en in klinische proeven.
