An Air-liquid Interface Bronchial Epithelial Model for Realistic, Repeated Inhalation Exposure to Airborne Particles for Toxicity Testing

Hedwig  M. Braakhuis; Ruiwen He; Rob  J. Vandebriel; Eric  R. Gremmer; Edwin Zwart; Jolanda  P. Vermeulen; Paul Fokkens; John Boere; Ilse Gosens; Flemming  R. Cassee

doi:10.3791/61210

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An Air-liquid Interface Bronchial Epithelial Model for Realistic, Repeated Inhalation Exposure to Airborne Particles for Toxicity Testing

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

An Air-liquid Interface Bronchial Epithelial Model for Realistic, Repeated Inhalation Exposure to Airborne Particles for Toxicity Testing

DOI:

10.3791/61210-v

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09:29 min

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May 13, 2020

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Hedwig M. Braakhuis, Ruiwen He², Rob J. Vandebriel, Eric R. Gremmer, Edwin Zwart, Jolanda P. Vermeulen, Paul Fokkens, John Boere, Ilse Gosens, Flemming R. Cassee²

¹National Institute for Public Health and the Environment (RIVM), ²Institute for Risk Assessment Sciences (IRAS)

Capítulos

00:04Introduction
00:54Seeding Calu-3 Cells onto Culture Inserts
03:09Preparing the Exposure Setup
05:14Handling the AES During an Exposure
07:02Results: Cell Model Selection
08:53Conclusion

Summary

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Described is the cell culture and exposure method of an in vitro bronchial model for realistic, repeated inhalation exposure to particles for toxicity testing.

An Air-liquid Interface Bronchial Epithelial Model for Realistic, Repeated Inhalation Exposure to Airborne Particles for Toxicity Testing

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Summary

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