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Biologia
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Simultaneous Isolation and Culture of Atrial Myocytes, Ventricular Myocytes, and Non-Myocytes from an Adult Mouse Heart
JoVE Journal
Biologia
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JoVE Journal Biologia
Simultaneous Isolation and Culture of Atrial Myocytes, Ventricular Myocytes, and Non-Myocytes from an Adult Mouse Heart

Simultaneous Isolation and Culture of Atrial Myocytes, Ventricular Myocytes, and Non-Myocytes from an Adult Mouse Heart

DOI:
10.3791/61224-v

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11:53 min

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June 14, 2020

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, , , ,
1San Diego State University Heart Institute and the Department of Biology,San Diego State University

Capítulos

  • 00:04Introduction
  • 01:29Surgical Procedure
  • 03:30Atrial Cell Isolation and Culture
  • 05:46Ventricular Cell Isolation and Culture
  • 07:46Stepwise Paradigm Calcium Reintroduction
  • 09:04Results: Representative Adult Mouse Heart Atrial and Ventricular Myocyte and Non-Myocyte Characterization
  • 11:09Conclusion

Summary

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A method is described for the simultaneous isolation of myocytes and non-myocytes from both the atria and ventricles of a single adult mouse heart. This protocol results in consistent yields of highly viable cardiac myocytes and non-myocytes and details optimal cell-specific culture conditions for phenotyping and in vitro analysis.

Tags

Cardiac Myocyte IsolationAdult Murine HeartAtrial MyocytesVentricular MyocytesNon-myocytesSimultaneous IsolationCell CultureAscending Aorta CannulationMicrosurgeryC57BL/6J Mouse

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