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前眼室人类胰岛移植和贡献宿主细胞的纵向维沃成像和定量

JoVE Journal
Medicina

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JoVE Journal Medicina

Longitudinal In Vivo Imaging and Quantification of Human Pancreatic Islet Grafting and Contributing Host Cells in the Anterior Eye Chamber

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前眼室人类胰岛移植和贡献宿主细胞的纵向维沃成像和定量

DOI:

10.3791/61234-v

•

09:07 min

•

June 11, 2020

•

Julia Nilsson², Dan Holmberg², Anja Schmidt-Christensen²

¹Department of Experimental Medical Science,Lund University, ²Lund University Diabetes Centre

Capítulos

00:04Introduction
01:08Transplantation Procedure
02:45Image Analysis
06:58Results: Image Segmentation of a Human Islet Grafted in the ACE of a NOD.ROSA-tomato.Rag^2-/- Recipient Mouse
08:17Conclusion

Summary

Tadução automática

该协议的目标是持续监测人类胰岛移植过程和贡献宿主与供体细胞的动态。这是通过移植人类小岛到NOD的眼部（ACE）前室来完成的。（Cg）-Gt（ROSA）26Sor^tm4-Rag2^-/-鼠标接收者，然后是重复的 2 光子成像。

Tags

In Vivo Imaging Pancreatic Islet Grafting Anterior Eye Chamber Beta Cell Replacement Therapy Islet Transplantation Two-photon Microscopy Islet Function And Viability Diabetic Complications Real-time Imaging

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