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Parallel høj gennemløb enkelt molekyle kinetisk analyse for site-specifikke DNA spaltning
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Biologia
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JoVE Journal Biologia
Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage

Parallel høj gennemløb enkelt molekyle kinetisk analyse for site-specifikke DNA spaltning

DOI:
10.3791/61236-v

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06:51 min

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May 06, 2020

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, ,
1Department of Biology,Emmanuel College, 2Department of Chemistry and Physics,Emmanuel College

Capítulos

  • 00:04Introduction
  • 00:59Making the Flow Cell
  • 02:10Tethering of DNA and Beads
  • 05:16Results: NdeI Cleavage is Dependent on Concentrations of Protein and Magnesium
  • 06:14Conclusion

Summary

Tadução automática

Tadução automática

En meget parallel metode til måling af den stedspecifikke kavalergang af DNA på et enkelt molekyleniveau er beskrevet. Denne protokol demonstrerer teknikken ved hjælp af begrænsningen endonuclease NDEI. Metoden kan nemt ændres til at studere enhver proces, der resulterer i sted-specifikke DNA spaltning.

Tags

DNA CleavageSingle Molecule KineticsHigh ThroughputDNA BindingDNA ModificationDNA Target SearchFlow CellTetheringPCRBuffer AAnti-digoxigeninFab Fragments

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