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来自卡诺哈布迪炎精英的蛋白质提取制剂制备和共免疫沉淀
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Protein Extract Preparation and Co-immunoprecipitation from Caenorhabditis elegans
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来自卡诺哈布迪炎精英的蛋白质提取制剂制备和共免疫沉淀

DOI:
10.3791/61243-v

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07:22 min

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May 23, 2020

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,
1Division of Biology,Kansas State University

Capítulos

  • 00:04Introduction
  • 01:05Worm Sample Collection
  • 01:59Worm Pellet Extract Preparation
  • 03:14Immunoprecipitation
  • 05:39Results: Representative Caenorhabditis elegans Protein Extract Co-Immunoprecipitation
  • 06:39Conclusion

Summary

Tadução automática

Tadução automática

该方法描述了从 卡诺哈布迪蒂斯精英 样本中制备高通量蛋白质提取物和随后的共免疫沉淀的协议。

Tags

Protein ExtractionCo-immunoprecipitationCaenorhabditis ElegansWorm Sample CollectionM9 BufferLysis BufferBead Mill HomogenizerProtein Extract PreparationImmunoprecipitationEndogenous ProteinsEndogenously Tagged ProteinsOverexpressed ProteinsGenetic Backgrounds

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