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Preparation of Mesh-Shaped Engineered Cardiac Tissues Derived from Human iPS Cells for In Vivo Myocardial Repair
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JoVE Journal Bioengenharia
Preparation of Mesh-Shaped Engineered Cardiac Tissues Derived from Human iPS Cells for In Vivo Myocardial Repair

Preparation of Mesh-Shaped Engineered Cardiac Tissues Derived from Human iPS Cells for In Vivo Myocardial Repair

DOI:
10.3791/61246-v

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05:05 min

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June 09, 2020

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, , , , ,
1Clinical Translational Research Program,RIKEN Center for Biosystems Dynamics Research, 2Department of Cardiovascular Surgery, Graduate School of Medicine,Kyoto University, 3Laboratory of Stem Cell and Neuro-Vascular Biology,Cell and Developmental Biology Center, National Institutes of Health, 4Kosair Charities Pediatric Heart Research Program, Cardiovascular Innovation Institute,University of Louisville, 5Department of Pediatrics, School of Medicine,University of Louisville, 6Department of Cardiovascular Surgery,Mitsubishi Kyoto Hospital, 7Cincinnati Children’s Heart Institute

Summary

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The present protocol generates mesh-shaped engineered cardiac tissues containing cardiovascular cells derived from human induced pluripotent stem cells to allow the investigation of cell implantation therapy for heart diseases.

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Human IPS CellsMyocardial RepairPDMS SheetPoloxamer 407Collagen Type IBasement Membrane MatrixCell CultureCardiac Regeneration

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