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脑毛细管中培养细胞细胞钙测量和钙成像研究
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Neurociência
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JoVE Journal Neurociência
Culture of Brain Capillary Pericytes for Cytosolic Calcium Measurements and Calcium Imaging Studies
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脑毛细管中培养细胞细胞钙测量和钙成像研究

DOI:
10.3791/61253-v

•

09:33 min

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May 27, 2020

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, ,
1Department of Pharmacy, The Faculty of Health and Medical Sciences,University of Copenhagen

Capítulos

  • 00:04Introduction
  • 00:41Isolation of Primary Pericytes from Bovine Brain Capillaries
  • 03:01Loading of Pericytes with Calcium Indicator Dye in a Plate Reader Setup
  • 05:18Live Imaging of Intracellular Ca2+ Levels
  • 07:11Results: Pericyte Isolation and Calcium Measurements
  • 09:02Conclusion

Summary

Tadução automática

Tadução automática

脑毛细管性信号是调节血脑屏障特性和血流的基本参与者。该协议描述了如何分离、培养脑毛细管腹膜，在细胞类型方面进行特征分析，并应用于用荧光探针研究细胞内钙信号。

Tags

Brain Capillary PericytesPrimary Cell CultureCalcium ImagingIntracellular SignalingCell IsolationCell DetachmentDMEMTrypsin-EDTACentrifugationCell ConfluencyMicroscopy

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