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测量小核苷酸基质的多核苷酸磷酸化的非放射性测定

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates

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测量小核苷酸基质的多核苷酸磷酸化的非放射性测定

DOI:

10.3791/61258-v

•

06:49 min

•

May 08, 2020

•

Monica C. Pillon, Robin E. Stanley

¹Signal Transduction Laboratory, National Institute of Environmental Health Sciences, Department of Health and Human Services,National Institutes of Health

Capítulos

00:05Introduction
00:29In Vitro RNA Kinase Reaction
01:27Gel Electrophoresis
04:53Results: Quantification of RNA Phosphorylation
06:06Conclusion

Summary

Tadução automática

该协议描述了一种非放射性检测，用于测量小DNA和RNA基质上多核苷酸激酶（PNKs）的激酶活性。

Tags

Keywords: Polynucleotide Kinase Phosphorylation DNA RNA Substrate Nonradioactive Assay Acrylamide Gel Denaturing Gel Urea TBE Buffer RNA Enzyme Kinase Reactions ATP Substrate Quenching Downstream Analysis

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