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Streamlined Sampling and Cultivation of the Pelagic Cosmopolitan Larvacean, Oikopleura dioica
JoVE Journal
Ambiente
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JoVE Journal Ambiente
Streamlined Sampling and Cultivation of the Pelagic Cosmopolitan Larvacean, Oikopleura dioica

Streamlined Sampling and Cultivation of the Pelagic Cosmopolitan Larvacean, Oikopleura dioica

DOI:
10.3791/61279-v

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11:55 min

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June 16, 2020

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, , , ,
1Okinawa Institute of Science and Technology Graduate University, 2Francis Crick Institute, 3UCL Genetics Institute, Department of Genetics, Evolution and Environment,University College London

Capítulos

  • 00:00Introduction
  • 00:45Initiation of Algal Culture
  • 02:41Field Collection of Oikopleura dioica
  • 04:04Isolation and Identification of O. dioica
  • 05:40O. dioica Culture Protocol
  • 07:38Preparation of Daily Food
  • 08:59Resultados
  • 10:23Conclusion

Summary

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Oikopleura dioica is a tunicate model organism in various fields of biology. We describe sampling methods, species identification, culturing setup, and culturing protocols for the animals and algal feed. We highlight key factors that helped strengthen the culture system and discuss the possible problems and resolutions.

Tags

Keywords: Oikopleura DioicaPelagic TunicateCulture SystemAlgal FoodChaetoceros CalcitransPlankton NetField SamplingMarine EcologyModel Organism

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