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Culturing Rat Sympathetic Neurons from Embryonic Superior Cervical Ganglia for Morphological and Proteomic Analysis
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JoVE Journal Neurociência
Culturing Rat Sympathetic Neurons from Embryonic Superior Cervical Ganglia for Morphological and Proteomic Analysis

Culturing Rat Sympathetic Neurons from Embryonic Superior Cervical Ganglia for Morphological and Proteomic Analysis

DOI:
10.3791/61283-v

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14:51 min

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September 27, 2020

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, ,
1Department of Biology,Saint Mary’s College of California

Capítulos

  • 00:00Introduction
  • 00:09Preparation of Media for Dissection and for Culturing Neurons
  • 00:42Preparation of Plates for Culturing Neurons
  • 01:56Dissection Set-up
  • 02:36Embryo Removal and Preparation
  • 04:17Isolation of the SCG from the Embryo
  • 06:52Post-processing of SCG
  • 11:00Maintenance of the Cultured SCG Neurons and Treatments
  • 13:02Representative Results- Immunostaining
  • 14:00Representative Results- Proteomic Analysis
  • 14:29Conclusion

Summary

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This paper describes the isolation and culturing of embryonic rat sympathetic neurons from the superior cervical ganglia. It also provides detailed protocols for immunocytochemical staining and for preparing neuronal extracts for mass spectrometric analysis.

Tags

Keywords: Rat Sympathetic NeuronsEmbryonic Superior Cervical GangliaMorphological AnalysisProteomic AnalysisCell CulturePoly-D-lysineDissection MediumControl MediumEnzymatic DigestionCentrifugationInstitutional Animal Care And Use Committee

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