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Culturing Rat Sympathetic Neurons from Embryonate Superior Cervical Ganglia for Morphological and Proteomic Analysis
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Capítulos
- 00:00Introduction
- 00:09Preparation of Media for Dissection and for Culturing Neurons
- 00:42Preparation of Plates for Culturing Neurons
- 01:56Dissection Set-up
- 02:36Embryo Removal and Preparation
- 04:17Isolation of the SCG from the Embryo
- 06:52Post-processing of SCG
- 11:00Maintenance of the Cultured SCG Neurons and Treatments
- 13:02Representative Results- Immunostaining
- 14:00Representative Results- Proteomic Analysis
- 14:29Conclusion
Cet article décrit l’isolement et la culture des neurones sympathiques embryonnaires de rat des ganglions cervicals supérieurs. Il fournit également des protocoles détaillés pour la coloration immunocytochimique et pour préparer des extraits neuronaux pour l’analyse spectrométrique de masse.
