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Optogenetic Activation of Afferent Pathways in Brain Slices and Modulation of Responses by Volatile Anesthetics
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Capítulos
- 00:04Introduction
- 00:45Preparation of Acute Brain Slices
- 02:47Placing Multi-Channel Probe in Ex Vivo Brain Tissue Slice
- 04:38Patch Clamping Targeted Neurons and Obtaining Whole-Cell Configuration
- 05:55Results: Intracellular and Multi-Channel Extracellular Recordings in Cortical Slice
- 07:19Conclusion
Ex vivo brain slices can be used to study the effects of volatile anesthetics on evoked responses to afferent inputs. Optogenetics are employed to independently activate thalamocortical and corticocortical afferents to non-primary neocortex, and synaptic and network responses are modulated with isoflurane.
