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Biologia
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Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue
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Biologia
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JoVE Journal Biologia
Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

DOI:
10.3791/61371-v

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05:46 min

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June 09, 2020

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, , , , , , , , , , , , ,
1Department of Medicine,Indiana University School of Medicine, 2Department of Cellular & Integrative Physiology,Indiana University School of Medicine, 3Division of Nephrology, Department of Medicine,Ohio State University Wexner Medical Center, 4Division of Pathology,Indiana University School of Medicine

Capítulos

  • 00:05Introduction
  • 00:41Cryosectioning and Laser Microdissection (LMD)
  • 03:39Results: Representative Human Kidney Regional Transcriptomics Analyses
  • 05:05Conclusion

Summary

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We describe a protocol for laser microdissection of sub-segments of the human kidney, including the glomerulus, proximal tubule, thick ascending limb, collecting duct and interstitium. The RNA is then isolated from the obtained compartments and RNA sequencing is carried out to determine changes in the transcriptomic signature within each sub-segment.

Tags

Laser MicrodissectionTranscriptomicsRegional AnalysisSingle Cell RNA SequencingCryosectioningImmunofluorescenceLaser Microdissection PlatformRNA IsolationLowly Expressed Transcripts

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