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Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells

Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells

DOI:
10.3791/61414-v

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09:02 min

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June 05, 2020

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, , , , , , , ,
1Stem Cell Research Center,Shantou University Medical College, 2The Center for Reproductive Medicine,Shantou University Medical College, 3Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology,Shantou University Medical College, 4Medical Research Center, Sun Yat-Sen Memorial Hospital,Sun Yat-Sen University, 5Department of Nucleic Acid Researches, Genetic Engineering and Biotechnology Research Institute,General Autority – City of Scientific Researches and Technological Applications

Capítulos

  • 00:04Introduction
  • 01:36HepG2.2.15 Cell Culture
  • 03:04Detection of HBV DNA in Supernatant Concentrate
  • 04:23In Vitro Infection of HepG2-NE and 293T-NE-3NRs Cells
  • 05:27HepG2.2.15 Co-culture with HepG2-NE / 293T-NE-3NRs / 293T-NE
  • 06:41Results: HBcAg Expression in 293T-NE, 293T-NE-3NRs and HepG2-NE
  • 07:47Conclusion

Summary

Tadução automática

Tadução automática

This manuscript describes a detailed protocol for Hepatitis B virus (HBV) infection in novel engineered 293T cells (293T-NE-3NRs, expressing human NTCP, HNF4α, RXRα and PPARα) and traditional hepatic cells (HepG2-NE, expressing human NTCP).

Tags

Hepatitis B VirusHBVNon-hepatic Cells293T-NE-3NRIn Vitro ModelHepG2.2.15Cell CultureViral ReplicationExtrahepatic SyndromesCell Line EngineeringBiosafetyViral SupernatantVirus ConcentrationReal-time PCR

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