Journal
/
Bioengenharia
/
Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells
JoVE Journal
Bioengenharia
This content is Free Access.
JoVE Journal Bioengenharia
Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

DOI:
10.3791/61433-v

•

09:20 min

•

August 11, 2020

•

, , , ,
1Department of Mechanical Engineering,University of Kentucky, 2Department of Chemistry,University of Kentucky, 3Department of Physiology,University of Kentucky, 4UK Light Microscopy Core,University of Kentucky

Capítulos

  • 00:04Introduction
  • 00:48Atomic Force Microscopy (AFM)
  • 04:32Confocal Microscopy
  • 06:42Clean Up
  • 07:14Results: Representative Conpokal Live Cell Imaging
  • 08:43Conclusion

Summary

Tadução automática

Tadução automática

Presented here is the protocol of the Conpokal technique, which combines confocal and atomic force microscopy into a single instrument platform. Conpokal provides same cell, same region, near simultaneous confocal imaging and mechanical characterization of live biological samples.

Tags

Keywords: ConpokalConfocal MicroscopyAtomic Force MicroscopyNear Simultaneous Dual-microscopyMechanobiologyAFM CantileverAFM ChipGlass BlockZ Stepper MotorBright-field MicroscopyManual MicrometersLookup TablesAFM Tip

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/61433?article_type=v

Bacterial Immobilization for Imaging by Atomic Force Microscopy
check_url/pt/61433?article_type=v

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy
check_url/pt/61433?article_type=v

Video-rate Scanning Confocal Microscopy and Microendoscopy
check_url/pt/61433?article_type=v

Quantifying the Mechanical Properties of the Endothelial Glycocalyx with Atomic Force Microscopy
check_url/pt/61433?article_type=v

Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy
check_url/pt/61433?article_type=v

A Novel Method for Localizing Reporter Fluorescent Beads Near the Cell Culture Surface for Traction Force Microscopy
check_url/pt/61433?article_type=v

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging
check_url/pt/61433?article_type=v

Novel Atomic Force Microscopy Based Biopanning for Isolation of Morphology Specific Reagents against TDP-43 Variants in Amyotrophic Lateral Sclerosis
check_url/pt/61433?article_type=v

Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers
check_url/pt/61433?article_type=v

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Read Article