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Assessing the Expression of Major Histocompatibility Complex Class I on Primary Murine Hippocampal Neurons by Flow Cytometry
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Assessing the Expression of Major Histocompatibility Complex Class I on Primary Murine Hippocampal Neurons by Flow Cytometry

Assessing the Expression of Major Histocompatibility Complex Class I on Primary Murine Hippocampal Neurons by Flow Cytometry

DOI:
10.3791/61436-v

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08:07 min

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May 19, 2020

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1Department of Biological Sciences,University of North Carolina at Charlotte

Summary

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This protocol describes a method for culturing primary hippocampal neurons from embryonic mouse brain. The expression of major histocompatibility complex class I on the extracellular surface of cultured neurons is then assessed by flow cytometric analysis.

Tags

Flow CytometryMHC Class IPrimary NeuronsHippocampusEmbryonic Brain DissectionPapain DissociationHibernate-E MediumTrituration

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