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Obtaining Human Microglia from Adult Human Brain Tissue
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JoVE Journal Neurociência
Obtaining Human Microglia from Adult Human Brain Tissue

Obtaining Human Microglia from Adult Human Brain Tissue

DOI:
10.3791/61438-v

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09:41 min

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August 30, 2020

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, , , ,
1Department of Bioscience and Bioengineering,Indian Institute of Technology Jodhpur, 2Department of Neurosurgery,All India Institute of Medical Sciences Jodhpur

Capítulos

  • 00:00Introduction
  • 01:06Tissue Acquisition and Processing: Day 0
  • 05:35Cell Culture: Day 2
  • 07:59Cell Culture: Day 4
  • 08:26Results: Representative Images of Adult Human Microglia
  • 09:15Conclusion

Summary

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This protocol is an efficient, cost effective and robust method of isolating primary microglia from live, adult, human brain tissue. Isolated primary human microglia can serve as a tool for studying cellular processes in homeostasis and disease.

Tags

Human MicrogliaPrimary Microglia CellsImmunocytochemistryCell IsolationTrypsin-EDTACentrifugationCell Culture

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