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Biologia do Desenvolvimento
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Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells

Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells

DOI:
10.3791/61446-v

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08:47 min

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May 15, 2020

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, , ,
1Departments of Biochemistry and Molecular Biology, Penn State College of Medicine,Penn State Hershey Cancer Institute

Capítulos

  • 00:04Introduction
  • 00:58EB, NPC, and Neuron Differentiation
  • 03:00Fixation and Embedding
  • 04:24Immunofluorescence
  • 05:45Results: E14 Differentiation into EBs, NPCs, and Neurons
  • 07:41Conclusion

Summary

Tadução automática

Tadução automática

We describe the procedure for the in vitro differentiation of mouse embryonic stem cells into neuronal cells using the hanging drop method. Furthermore, we perform a comprehensive phenotypic analysis through RT-qPCR, immunofluorescence, RNA-seq, and flow cytometry.

Tags

Keywords: Mouse Embryonic Stem CellsNeuron DifferentiationNeural ProgenitorsEctodermal DevelopmentHanging Drop CultureEmbryoid BodyNeural Progenitor CellsRetinoic AcidImmunocytochemistry

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