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大规模混合阶段 卡诺哈布迪蒂斯电子 人种群的文化和分析

JoVE Journal
Genética

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JoVE Journal Genética

Culture and Assay of Large-Scale Mixed-Stage Caenorhabditis elegans Populations

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大规模混合阶段卡诺哈布迪蒂斯电子人种群的文化和分析

DOI:

10.3791/61453-v

•

08:51 min

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May 05, 2021

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Amanda O. Shaver, Goncalo J. Gouveia, Pamela S. Kirby, Erik C. Andersen, Arthur S. Edison^2,3

¹Department of Genetics,University of Georgia, ²Department of Biochemistry and Molecular Biology,University of Georgia, ³Complex Carbohydrate Research Center,University of Georgia, ⁴Department of Molecular Biosciences,Northwestern University

Capítulos

00:04Introduction
00:28Large-Scale Culture Plate (LSCP) Gravid Adult Spot Bleaching
01:21LSCP Sample Harvest
03:02Population Size Estimation
04:19Large Particle Flow Cytometry (LPFC) Preparation
05:27Population Distribution Documentation and 384-Well Imaging Plate Preparation
06:24Results: Representative Large-Scale Mixed-Stage C. elegans Population Evaluation
08:06Conclusion

Summary

Tadução automática

要在经济学研究中使用卡诺哈布迪炎（C.elegans），需要一种方法来生成大量蠕虫，其中单个样本可以在平台上测量以进行比较分析。在这里，提出了一种在大型文化板块（LSCPs）上培养C.elegans种群并记录人口增长的方法。

Tags

Keywords: Caenorhabditis Elegans Large-scale Culture Mixed-stage Populations Omics Experiments Spot Bleaching Alkaline Hypochlorite Solution M9 Solution Worm Suspension Population Size Estimation

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