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Stratégie phosphoproteomic pour profiler la signalisation osmotique de stress dans Arabidopsis
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1Department of Plant Biology,Carnegie Institute for Science, 2Graduate School of Pharmaceutical Sciences,Kyoto University, 3Department of Biochemistry,Purdue University, 4Department of Chemistry,Purdue University, 5Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences
Capítulos
- 00:04Introduction
- 00:33Immobilized Metal Ion Affinity Chromatography (IMAC) and High pH Reversed-Phase (Hp-RP) Stage Tip Preparation
- 01:10IMAC Stage Tip Phosphopeptide Enrichment
- 02:44Hp-RP C18 Stage Tip Phosphopeptide Fractionation
- 03:37Results: Representative Osmotic Stress Signaling in Arabidopsis
- 05:05Conclusion
Présenté ici est une approche phosphoproteomic, à savoir arrêter et aller pointe d’extraction à base de phosphoproteomic, qui fournit un haut débit et une couverture profonde du phosphoprotéome Arabidopsis. Cette approche délimite la vue d’ensemble de la signalisation osmotique de stress dans Arabidopsis.
