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Direct-Coupled Electroretinogram (DC-ERG) for Recording the Light-Evoked Electrical Responses of the Mouse Retinal Pigment Epithelium
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Direct-Coupled Electroretinogram (DC-ERG) for Recording the Light-Evoked Electrical Responses of the Mouse Retinal Pigment Epithelium

Direct-Coupled Electroretinogram (DC-ERG) for Recording the Light-Evoked Electrical Responses of the Mouse Retinal Pigment Epithelium

DOI:
10.3791/61491-v

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09:10 min

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July 14, 2020

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, , , ,
1Retinal Neurophysiology Section, National Eye Institute,National Institutes of Health, 2Ocular and Stem Cell Translational Research Section, National Eye Institute,National Institutes of Health, 3Human Visual Function Core, National Eye Institute,National Institutes of Health

Capítulos

  • 00:04Introduction
  • 00:44Capillary Electrode Filling
  • 01:51Microelectrode Holder Preparation
  • 02:46Electrode Test
  • 04:08Mouse and Electrode Positioning
  • 06:15Direct-Coupled Electroretinogram (DC-ERG) Recording
  • 07:04Results: Representative DC-ERG Traces and Workflow Analyses
  • 08:25Conclusion

Summary

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Here, we present a method for recording light-evoked electrical responses of the retinal pigment epithelium (RPE) in mice using a technique known as DC-ERGs first described by Marmorstein, Peachey, and colleagues in the early 2000s.

Tags

Direct-coupled ElectroretinogramDC-ERGRetinal Pigment EpitheliumNon-invasive EvaluationAge-related ChangesDisease ProgressionPharmacological InterventionReproducibilityCapillary ElectrodeHBSSVacuum ChamberCustom StandMicroelectrode HolderT-clipMagnetic Ball JointElectrode TestGround ElectrodeReference Electrode

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