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En Patient-Afledt Xenograft Model for Venøs misdannelser
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Developmental Biology
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JoVE Journal Developmental Biology
A Patient-Derived Xenograft Model for Venous Malformation

En Patient-Afledt Xenograft Model for Venøs misdannelser

DOI:
10.3791/61501-v

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06:51 min

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June 15, 2020

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, ,
1Division of Experimental Hematology and Cancer Biology,Cincinnati Children’s Hospital Medical Center, 2Department of Pediatrics,University of Cincinnati College of Medicine

Chapters

  • 00:04Introduction
  • 00:42VM Patient-derived Xenograft Protocol
  • 03:11Tissue Collection and Processing
  • 03:45Analysis of Human-derived Vascular Channels
  • 05:13Results: VM Lesion Plug
  • 06:10Conclusion

Summary

Automatic Translation

Automatic Translation

Vi præsenterer en detaljeret protokol til at generere en murine xenograft model af venøs misdannelser. Denne model er baseret på subkutan injektion af patientbaserede endotelceller, der indeholder hyperaktiverende TIE2- og/eller PIK3CA-genmutationer. Xenograft læsioner nøje opsummere de histopatologiske træk ved VM patientvæv.

Tags

Patient-derived XenograftVenous MalformationPreclinical Therapeutic TestingEndothelial CellsVascular AnomaliesTrypsinizationCell SuspensionCentrifugationBMEMAnesthesiaInjectionCaliper MeasurementFormalin Fixation

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