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预测使用局部循环单核祖细胞在血管成形术治疗的危肢缺血患者截肢

JoVE Journal
Medicina

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JoVE Journal Medicina

Predicting Amputation using Local Circulating Mononuclear Progenitor Cells in Angioplasty-treated Patients with Critical Limb Ischemia

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预测使用局部循环单核祖细胞在血管成形术治疗的危肢缺血患者截肢

DOI:

10.3791/61503-v

•

07:25 min

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September 22, 2020

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Juan Antonio Suárez-Cuenca, Eduardo Vera-Gómez, Alejandro Hernández-Patricio, Atzín Suá Ruíz-Hernández, Juan Ariel Gutiérrez-Buendía, Carlos Ramiro Zamora-Alemán, Alberto Melchor-López, Yasser Alberto Rizo-García, Oscar Antonio Lomán-Zúñiga, Ignacio Escotto-Sánchez, Juan Miguel Rodríguez-Trejo, Rebeca Pérez-Cabeza de Vaca, Mario Antonio Téllez-González, Paul Mondragón-Terán

¹Experimental Metabolism and Clinical Research Laboratory, Clinical Research Department, Division of Biomedical Research,Centro Médico Nacional 20 de Noviembre, ISSSTE, ²Vascular Surgery and Angiology Department,Centro Médico Nacional 20 de Noviembre, ISSSTE, ³Regenerative Medicine and Tissue Engineering Laboratory; Coordination of Research,Centro Médico Nacional 20 de Noviembre, ISSSTE

Capítulos

00:04Introduction
01:33Flow-Mediated Dilation (FMD) Evaluation
02:11Lower Limb Vascular Blood Evaluation, Blood Sampling, and Balloon Angioplasty
04:07Circulating Mononuclear Progenitor Cell (MPC) Isolation and Antibody Staining
05:21MPC Quantification
06:10Results: Representative Relationship Between MPC and Hemodynamic Indicators and Lower Limb Amputation Prognosis After Angioplasty
07:04Conclusion

Summary

Tadução automática

即使严重肢体缺血（CLI）中受阻血管成形术，下肢截肢也可能发生。单核先天细胞（MPC）反映血管修复。本协议描述了从接近血管成形术的循环中量化MPC，以及它与内皮功能障碍和下肢截肢预测的关系。

Tags

Keywords: – Angioplasty – Critical Limb Ischemia – Limb Amputation – Mononuclear Progenitor Cells (MPCs) – Endothelial Dysfunction – Vascular Events – Ischemic Heart Disease – Lower Limb Ischemia – Risk Stratification – Rutherford Classification – Brachial Artery Diameter – Flow-mediated Dilation (FMD) – Vascular Obstruction – Angioplasty Balloon Catheter

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