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Biologia do Desenvolvimento
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Minimum Volume Vitrification of Immature Feline Oocytes
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Minimum Volume Vitrification of Immature Feline Oocytes

Minimum Volume Vitrification of Immature Feline Oocytes

DOI:
10.3791/61523-v

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07:16 min

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June 24, 2020

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1Dipartimento di Scienze Veterinarie per la Salute, la Produzione Animale e la Sicurezza Alimentare ‘Carlo Cantoni’,Università degli Studi di Milano

Capítulos

  • 00:04Introduction
  • 00:52Vitrification
  • 03:38Warming
  • 05:35Results: Viability of Vitrified Cat Oocytes after Warming
  • 06:33Conclusion

Summary

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This manuscript describes a protocol for the minimum volume vitrification of immature cat oocytes with laboratory-made media on commercial supports. It covers every step from oocyte isolation from ex vivo gonads to vitrification and warming.

Tags

Feline OocytesVitrificationCryopreservationFertility PreservationBiodiversityDomestic CatGamete BiobankCOCsEquilibration SolutionVitrification SolutionLiquid Nitrogen

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