É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:04Introduction
- 00:33Migration Plate Preparation
- 01:11Scratch Migration Assay
- 02:13Fixation
- 03:28Image Analysis
- 05:20Migrating Cell Quantification and Migration Area Determination
- 06:56Results: Representative Scratch Migration Assay Analyses
- 08:15Conclusion
We present a cost-effective method to the scratch migration assay that provides a new approach for determining cell migration without the use of equipment-intensive methods. While fibroblasts were used in this protocol, it can be adapted and utilized to study additional cell types and influences on cell migration.
