/
/
Detektion av mikroRNA-uttryck i njurarna av immunglobulin, en nefropatisk möss
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
1Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center,Jichi Medical University, 2Division of Intensive Care Unit, First Department of Integrated Medicine, Saitama Medical Center,Jichi Medical University, 3Department of Medical Physiology,Meiji Pharmaceutical University
Capítulos
- 00:04Introduction
- 00:43Obtaining Kidney Samples from HIGA Mice
- 01:27Total RNA Purification from Kidney Samples
- 02:14cDNA Synthesis and qRT‐PCR of miRNA
- 03:55Results: Expression Levels of miRNAs in the Kidneys of HIGA Mice
- 04:57Conclusion
mikroRNA är involverade i patogenesen av IgA-nefropati. Vi har utvecklat en tillförlitlig metod för att detektera mikroRNA-uttrycksnivåer i njurarna hos en IgA-nefropatimusmodell (HIGA-möss). Denna nya metod kommer att underlätta för att kontrollera miRNAs engagemang i IgA-nefropati.
