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Måling naturligt erhvervet fagcytose-inducerende antistoffer mod plasmodium falciparum parasitter ved en Flow Cytometri-Baseret Analyse
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1Centre for Medical Parasitology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences,University of Copenhagen, 2West African Centre for Cell Biology of Infectious Pathogens, Department of Biochemistry, Cell and Molecular Biology,University of Ghana, 3Department of Immunology,Noguchi Memorial Institute for Medical Research, 4Centre for Medical Parasitology, Department of Infectious Diseases,Rigshospitalet
Capítulos
- 00:04Introduction
- 00:33THP-1 and Infected Erythrocyte (IE) Culture Setup
- 01:58Parasite Staining and Opsonization Plate Preparation
- 03:47THP-1 Cell and Phagocytosis Plate Preparation
- 04:35Phagocytosis Assay
- 06:21Flow Cytometry Acquisition and Analysis
- 07:34Results: Representative Evaluation of Parasite-IE Opsonization and Phagocytosis
- 09:21Conclusion
Det overordnede mål med denne protokol er at give instruktion om, hvordan man måler kapaciteten af antistoffer til stede i sera eller plasma af personer, naturligt udsat for Plasmodium falciparum infektion, at opsonisere og fremkalde fagocytose af parasit-inficerede erythrocytter (IEs).
