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Mätning Naturligt förvärvade Phagocytosis-inducerande antikroppar mot Plasmodium falciparum parasiter av en Flow Cytometry-baserade assay
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1Centre for Medical Parasitology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences,University of Copenhagen, 2West African Centre for Cell Biology of Infectious Pathogens, Department of Biochemistry, Cell and Molecular Biology,University of Ghana, 3Department of Immunology,Noguchi Memorial Institute for Medical Research, 4Centre for Medical Parasitology, Department of Infectious Diseases,Rigshospitalet
Capítulos
- 00:04Introduction
- 00:33THP-1 and Infected Erythrocyte (IE) Culture Setup
- 01:58Parasite Staining and Opsonization Plate Preparation
- 03:47THP-1 Cell and Phagocytosis Plate Preparation
- 04:35Phagocytosis Assay
- 06:21Flow Cytometry Acquisition and Analysis
- 07:34Results: Representative Evaluation of Parasite-IE Opsonization and Phagocytosis
- 09:21Conclusion
Det övergripande målet med detta protokoll är att ge instruktioner om hur man mäter kapaciteten hos antikroppar som finns i sera eller plasma av individer, naturligt utsatt för Plasmodium falciparum infektion, att opsonize och inducera fagocytos av parasitinfekterade erytrocyter (IEs).
