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1Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen University Health Science Center,Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, 2Xianning Hospital of Traditional Chinese Medicine, 3Liver-biotechnology (Shenzhen) Co., Ltd., 4Department of Radiology,Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, 5Department of Life Sciences,University of Toronto, 6Jiangsu Key Laboratory of Xenotransplantation,Nanjing Medical University, 7Department of Hepatopancreatobiliary Surgery,Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital
Capítulos
- 00:04Introduction
- 00:52Isolating Genomic DNA
- 02:20Verification of Primer Specificity
- 03:42Standard Curve of cpsDNA
- 04:45Quantitation of Circulating Pig-specific DNA
- 05:55Results: PCR Validation of Porcine Specific Primers
- 06:51Conclusion
Dans ce protocole, des amorces spécifiques de porcin ont été conçues, des fragments spécifiques d’ADN de porcine contenant des plasmides ont été construits, et des courbes standard pour la quantitation ont été établies. À l’aide d’amorces spécifiques aux espèces, cpsDNA a été quantifié par qPCR dans des modèles de transplantation de cellules de porc à souris et des modèles de transplantation de patchs d’artère de porc à singe.
