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Analyzing the α-Actinin Network in Human iPSC-Derived Cardiomyocytes Using Single Molecule Localization Microscopy
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Biologia
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JoVE Journal Biologia
Analyzing the α-Actinin Network in Human iPSC-Derived Cardiomyocytes Using Single Molecule Localization Microscopy

Analyzing the α-Actinin Network in Human iPSC-Derived Cardiomyocytes Using Single Molecule Localization Microscopy

DOI:
10.3791/61605-v

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07:02 min

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November 03, 2020

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, , , ,
1Department of Cardiac Surgery, Reference and Translation Center for Cardiac Stem Cell Therapy (RTC),Rostock University Medical Center, 2Faculty of Interdisciplinary Research, Department Life, Light & Matter,University Rostock, 3Department of Cardiology,Rostock University Medical Center

Capítulos

  • 00:04Introduction
  • 00:27Photoactivated Localization Microscopy (PALM) Image Acquisition Setup
  • 01:27PALM Image Acquisition
  • 01:50PALM Data Reconstruction
  • 02:39Reconstructed PALM Image Post Processing
  • 03:34Sarcomere Filament Analysis
  • 04:41Results: Representative Human Induced Pluripotent Stem Cell (iPSC)-Derived Cardiomyocyte alpha-Actinin Network Analysis
  • 06:23Conclusion

Summary

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The formation of a proper sarcomere network is important for the maturation of iPSC-derived cardiomyocytes. We present a super resolution-based approach that allows for the quantitative evaluation of the structural maturation of stem cell derived cardiomyocytes, to improve culture conditions promoting cardiac development.

Tags

Keywords: Single Molecule Localization MicroscopyIPSC-derived Cardiomyocytesα-ActininSarcomere MaturationSuper-resolution ImagingPALM ImagingTIRF MicroscopyImageJThunderstorm PluginSarcomere Length Analysis

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