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Analyse af α-Actinin Network i Human iPSC-Afledte Kardiomyocytter ved hjælp af enkelt molekyle lokalisering mikroskopi
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Biologia
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JoVE Journal Biologia
Analyzing the α-Actinin Network in Human iPSC-Derived Cardiomyocytes Using Single Molecule Localization Microscopy

Analyse af α-Actinin Network i Human iPSC-Afledte Kardiomyocytter ved hjælp af enkelt molekyle lokalisering mikroskopi

DOI:
10.3791/61605-v

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07:02 min

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November 03, 2020

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, , , ,
1Department of Cardiac Surgery, Reference and Translation Center for Cardiac Stem Cell Therapy (RTC),Rostock University Medical Center, 2Faculty of Interdisciplinary Research, Department Life, Light & Matter,University Rostock, 3Department of Cardiology,Rostock University Medical Center

Capítulos

  • 00:04Introduction
  • 00:27Photoactivated Localization Microscopy (PALM) Image Acquisition Setup
  • 01:27PALM Image Acquisition
  • 01:50PALM Data Reconstruction
  • 02:39Reconstructed PALM Image Post Processing
  • 03:34Sarcomere Filament Analysis
  • 04:41Results: Representative Human Induced Pluripotent Stem Cell (iPSC)-Derived Cardiomyocyte alpha-Actinin Network Analysis
  • 06:23Conclusion

Summary

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Tadução automática

Dannelsen af et ordentligt sarkomnetværk er vigtig for modningen af iPSC-afledte kardiomyocytter. Vi præsenterer en super opløsning-baseret tilgang, der giver mulighed for kvantitativ evaluering af den strukturelle modning af stamceller afledte kardiomyocytter, for at forbedre kultur betingelser fremme hjerteudvikling.

Tags

Single Molecule Localization MicroscopyIPSC-derived Cardiomyocytesα-ActininSarcomere MaturationSuper-resolution ImagingPALM ImagingTIRF MicroscopyImageJThunderstorm PluginSarcomere Length Analysis

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