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血液由来神経細胞のGMフリー生成
JoVE Journal
Neurociência
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JoVE Journal Neurociência
GM-Free Generation of Blood-Derived Neuronal Cells
DOI:

08:11 min

February 13, 2021

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Capítulos

  • 00:04Introduction
  • 00:47Isolation of Human Peripheral Blood Mononuclear Cells (PBMNCs
  • 01:25Activation of Human GPI-Anchored Glycoprotein by Antibody Crosslinking on the Surface of PBMNCs
  • 02:21Sorting of Newly Generated Dedifferentiated Cells
  • 03:33Preparing Cell Culture Dishes for Neuronal Differentiation of Newly Generated Stem Cells
  • 04:10Culturing of Neuronal Differentiated Blood Cells
  • 04:44Immunofluorescence Microscopy Analysis of Blood-Derived Neural Cells
  • 06:08Results: Morphology, Re-differentiation and Immunophenotyping of Generated Neuronal Cells
  • 07:41Conclusion

Summary

Tadução automática

我々は、再プログラム化された末梢血細胞から神経表現型を有する細胞を得るための遺伝子組換え非遺伝子組換え(GMフリー)法を提示する。新規ヒトGPI結合タンパク質にリンクされたシグナル伝達経路の活性化は、ヒト多能性幹細胞を得るための効率的なGMフリーの方法を明らかにする。

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