Preparing and Injecting Embryos of <em>Culex</em> Mosquitoes to Generate Null Mutations using CRISPR/Cas9

Megan E. Meuti; Robert Harrell

doi:10.3791/61651

Journal

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Biologia

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Preparing and Injecting Embryos of Culex Mosquitoes to Generate Null Mutations using CRISPR/Cas9

JoVE Journal
Biologia

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JoVE Journal Biologia

Preparing and Injecting Embryos of Culex Mosquitoes to Generate Null Mutations using CRISPR/Cas9

Preparing and Injecting Embryos of Culex Mosquitoes to Generate Null Mutations using CRISPR/Cas9

DOI:

10.3791/61651-v

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07:45 min

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September 10, 2020

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Megan E. Meuti, Robert Harrell

¹Department of Entomology,The Ohio State University, ²Insect Transformation Facility, Institute for Bioscience and Biotechnology Research,University of Maryland

Capítulos

00:04Introduction
01:10Injecting Culex Embryos
05:44Results: Transmission of the Desired Mutation
06:42Conclusion

Summary

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CRISPR/Cas9 is increasingly used to characterize gene function in non-model organisms. This protocol describes how to generate knock-out lines of Culex pipiens, from preparing injection mixes, to obtaining and injecting mosquito embryos, as well as how to rear, cross, and screen injected mosquitoes and their progeny for desired mutations.

Preparing and Injecting Embryos of Culex Mosquitoes to Generate Null Mutations using CRISPR/Cas9

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