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使用免疫增强技术的超高速西布洛特

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology

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使用免疫增强技术的超高速西布洛特

DOI:

10.3791/61657-v

•

05:59 min

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September 26, 2020

•

Sayuri L. Higashi², Kazuya Yagyu², Haruna Nagase², Craig S. Pearson, Herbert M. Geller, Yasuhiro Katagiri

¹Laboratory of Developmental Neurobiology, Cell and Developmental Biology Center, National Heart, Lung, and Blood Institute,National Institutes of Health, ²United Graduate School of Drug Discovery and Medical Information Sciences,Gifu University

Capítulos

00:05Introduction
00:51Incubation with Primary Antibody
01:59Membrane Wash
02:59Image Acquisition
03:52Results: Optimization and Validation of CDR Method in conjunction with IRE
05:22Conclusion

Summary

Tadução automática

通过循环排泄和补充（CDR）技术与免疫增异剂一起改进抗原抗体结合的动能，开发出一种超高速西方印迹技术。

Tags

Keywords: Ultra-high-speed Western Blot Immunoreaction Enhancing Technology PVDF Membrane Primary Antibody Secondary Antibody Chemiluminescent Substrate Salad Spinner Cyclic Draining And Replenishing Biomedical Research Time-saving

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