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Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
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Biologia
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JoVE Journal Biologia
Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

DOI:
10.3791/61665-v

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07:43 min

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October 06, 2020

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, , , ,
1Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology,Shanghai Jiao Tong University, 2College of Agronomy,Anhui Agricultural University, Heifei, China, 3School of Agriculture, Food and Wine,University of Adelaide

Capítulos

  • 00:04Introduction
  • 00:54Rice Genetic Transformation and Plant Tissue Culture
  • 04:00Observation of the Basic Phenotype of the Mutant
  • 05:14Results: Targeted Mutagenesis of the OsABCG15
  • 07:16Conclusion

Summary

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This work describes the use of CRISPR-Cas9 genome editing technology to knockout endogenous gene OsABCG15 followed by a modified Agrobacterium-mediated transformation protocol to produce a stable male-sterile line in rice.

Tags

Keywords: Agrobacterium-mediated TransformationTransgenic ProductionRiceMale Reproductive DevelopmentYoung InflorescencesCallus ProductionGene TransformationRegenerationVideo DemonstrationMeiosis StageNBD2 MediumEB PlateYEB MediumAAM AS MediumEmbryogenic Calli

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