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Aislamiento de células espermatogénicas murinas usando un tinte de unión de ADN permeable a células violetas-excitado
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1Division of Reproductive Sciences, Division of Developmental Biology, Perinatal Institute,Cincinnati Children’s Hospital Medical Center, 2Department of Pediatrics,University of Cincinnati College of Medicine, 3Division of Germ Cell Biology, National Institute for Basic Biology,National Institutes of Natural Sciences, 4Department of Basic Biology, School of Life Science,Graduate University for Advanced Studies (Sokendai), 5Department of Animal Science and Biotechnology, School of Veterinary Medicine,Azabu University, 6Faculty of Science and Technology, Department of Applied Biological Science,Tokyo University of Science
Capítulos
- 00:04Introduction
- 00:53Testicular Cell Suspension Preparation
- 02:35Cell Staining
- 03:30Flow Cytometry
- 07:08Results: Representative Spermatogenic Cell Imaging, Yield, and Viability
- 07:55Conclusion
Aquí presentamos un método simple y eficiente para aislar células germinales meíticas y post-meyóticas vivas de los testículos de ratón adultos. Usando un tinte de unión de ADN excitado por violeta y clasificación celular activada por fluorescencia, se pueden aislar poblaciones de células espermatogénicas altamente enriquecidas para muchas aplicaciones posteriores.
