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Demonstration of Heterologous Complexes formed by Golgi-Resident Type III Membrane Proteins using Split Luciferase Complementation Assay
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Biologia
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JoVE Journal Biologia
Demonstration of Heterologous Complexes formed by Golgi-Resident Type III Membrane Proteins using Split Luciferase Complementation Assay

Demonstration of Heterologous Complexes formed by Golgi-Resident Type III Membrane Proteins using Split Luciferase Complementation Assay

DOI:
10.3791/61669-v

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05:28 min

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September 10, 2020

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, ,
1Laboratory of Biochemistry, Faculty of Biotechnology,University of Wroclaw

Capítulos

  • 00:04Introduction
  • 00:38Transient Transfection of the Expression Plasmids into the Cells
  • 02:06Measuring Luminescence
  • 02:57Results: Split Luciferase Complementation Assay Performed in HEK293T
  • 04:46Conclusion

Summary

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The protocol presented here is intended to demonstrate the occurrence of heterologous interactions between Golgi-resident type III membrane proteins with cytoplasmically exposed N- and/or C-termini in live mammalian cells using the most recent variant of the split luciferase complementation assay.

Tags

Keywords: Heterologous ComplexesGolgi-resident Type III Membrane ProteinsSplit Luciferase Complementation AssayHEK293 T-cellsTransfectionLuminescenceNucleotide Sugar TransportersSLC35A2SLC35A3Protein-protein Interactions

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