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Neutron Radiography and Computed Tomography of Biological Systems at the Oak Ridge National Laboratory’s High Flux Isotope Reactor
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Biologia
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JoVE Journal Biologia
Neutron Radiography and Computed Tomography of Biological Systems at the Oak Ridge National Laboratory’s High Flux Isotope Reactor

Neutron Radiography and Computed Tomography of Biological Systems at the Oak Ridge National Laboratory’s High Flux Isotope Reactor

DOI:
10.3791/61688-v

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10:24 min

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May 07, 2021

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, , , , , , , , , ,
1Neutron Scattering Division,Oak Ridge National Laboratory, 2College of Veterinary Medicine,The University of Tennessee, 3UT-ORNL Graduate School of Genome, Science and Technology,The University of Tennessee, 4Integrity Laboratories, 5Environmental Sciences Division,Oak Ridge National Laboratory, 6Department of Cell & Molecular Medicine, Rush Medical College,Rush University, 7Computer Science and Mathematics Division,Oak Ridge National Laboratory, 8Electrification and Energy Infrastructures Division,Oak Ridge National Laboratory, 9Now at Second Target Station Project,Oak Ridge National Laboratory, 10Neutron Technologies Division,Oak Ridge National Laboratory

Capítulos

  • 00:05Introduction
  • 01:17Instrument Setup
  • 03:52Data Acquisition
  • 06:32Volume Reconstruction and Data Processing
  • 08:01Results: Neutron Imaging of a Rat Femur, Mouse Lung, and Plant Root/Soil System
  • 09:25Conclusion

Summary

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This manuscript describes a protocol for neutron radiography and computed tomography of biological samples using a High Flux Isotope Reactor (HFIR) CG-1D beamline to measure a metal implant in a rat femur, a mouse lung, and an herbaceous plant root/soil system.

Tags

Keywords: Neutron RadiographyComputed TomographyBiological SystemsOak Ridge National LaboratoryHigh Flux Isotope ReactorNon-destructiveNon-invasiveHydrogen ContentWater ContentPlant RootsSoilEnergy MaterialsMaterial ScienceEngenhariaPlantsSoilWater MovementsTumor MarginsSmall Resected Intact TumorsNeutron ScatteringImagingAPEX Imaging InterfaceRadiographsResolution MaskDetector MotorSample RotationSample ContainerAluminum Foil

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