Journal
/
Biologia
/
Isolation, Proliferation and Differentiation of Rhesus Macaque Adipose-Derived Stem Cells
JoVE Journal
Biologia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
Isolation, Proliferation and Differentiation of Rhesus Macaque Adipose-Derived Stem Cells

Isolation, Proliferation and Differentiation of Rhesus Macaque Adipose-Derived Stem Cells

DOI:
10.3791/61732-v

•

07:33 min

•

May 26, 2021

•

, ,
1Department of Physiology,Louisiana State University Health Sciences Center – New Orleans, 2Comprehensive Alcohol-HIV/AIDS Research Center,Louisiana State University Health Sciences Center – New Orleans

Capítulos

  • 00:04Introduction
  • 00:34Isolation of Adipose-Derived Stem Cells-ADSC
  • 04:02ADSC Proliferation
  • 05:23Adipogenic Differentiation
  • 06:01Results: Observation of ADSCs Using Light Microscopy
  • 06:45Conclusion

Summary

Tadução automática

Tadução automática

In this article, we describe isolation of rhesus macaque derived adipose-derived stem cells (ADSCs) using an enzymatic tissue digestion protocol. Next, we describe ADSC proliferation which includes cell detachment, counting and plating. Lastly, ADSC differentiation is described using specific adipogenic inducing agents. Additionally, we describe staining techniques to confirm differentiation.

Tags

Adipose-derived Stem CellsRhesus MacaqueIsolationProliferationDifferentiationMetabolic AssaysInsulin-stimulated Glucose UptakeLipogenesisLipolysisCollagenase DigestionRBC LysisCell Culture

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Isolation and Transplantation of Hematopoietic Stem Cells (HSCs)

Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells

Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates

Isolation, Characterization, and Differentiation of Cardiac Stem Cells from the Adult Mouse Heart

Author Spotlight: Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats

Author Spotlight: Advancing Treatment Approaches with Adipose-Derived Stem Cells

Author Spotlight: Advancements in Stem Cell Regenerative Therapy Through Photobiomodulation

Murine Adipose-Derived Mesenchymal Cell Isolation, Maintenance and Characterization

Immunofluorescent Detection of Adipose-Derived Stem Cell Expression Markers

Read Article