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Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability
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Biologia
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JoVE Journal Biologia
Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability

Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability

DOI:
10.3791/61733-v

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06:42 min

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September 01, 2020

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, , ,
1Department of Biophysics,University of Texas Southwestern Medical Center, 2Center for Alzheimer’s and Neurodegenerative Disease and Center for Systems Biology,University of Texas Southwestern Medical Center

Capítulos

  • 00:04Introduction
  • 00:49Cryopreservation of Mammalian Cells in an NMR Rotor
  • 01:25Cryogenic Transfer of a Frozen Sample into the NMR Spectrometer
  • 03:48Cryogenic Removal of the Sample from the NMR Spectrometer
  • 05:02Results: Cryogenic Insertion of a Pre-frozen Sample of Mammalian Cells Results in Cellular Intactness Similar to Never-frozen Mammalian Cell Samples
  • 05:47Conclusion

Summary

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Presented here is a protocol for cryogenic transfer of frozen samples into the dynamic nuclear polarization (DNP) magic angle spinning (MAS) nuclear magnetic resonance (NMR) probe. The protocol includes directions for rotor storage prior to the experiment and directions for viability measurements before and after the experiment.

Tags

Keywords: Cryogenic Sample LoadingMagic Angle SpinningNuclear Magnetic ResonanceSpectrometerCellular ViabilityDNP Assisted MAS NMRProtein CharacterizationSample HandlingCryogenic TransferRotorLiquid NitrogenMicrocentrifuge TubeNMR Sample CatcherSpectrometer Facility

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