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使用高通量微流体设备生成动态环境条件

JoVE Journal
Bioengenharia
Author Produced

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JoVE Journal Bioengenharia

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

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使用高通量微流体设备生成动态环境条件

DOI:

10.3791/61735-v

•

14:48 min

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April 17, 2021

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Bingchen Che*¹, Jie Zhu*¹, Dan Sun, Xiaoqiang Feng, Ce Zhang

¹State Key Laboratory of Cultivation Base for Photoelectric Technology and Functional Materials, State Key Laboratory of Photon-Technology in Western China Energy, Institute of Photonics and Photon-Technology,Northwest University

Capítulos

00:00Introduction
02:23Microfluidic Chip Design
04:33Chip Fabrication and Operation
05:32Microfluidic Chip Production using Soft Lithograph
07:03Chip Operation
07:59Generation of Dynamic Inputs in Cellular Microenviroments
08:43Setup for High Throughput Live-cell Imaging
10:44Automatic Data Analysis
11:42Discussion

Summary

Tadução automática

我们介绍了一个用于复杂生命机械高吞吐量研究的微流体系统，该系统由 1500 个培养单元、一系列增强的永久泵和现场混合模组组成。微流体芯片可分析体内高度复杂和动态的微环境条件。

Tags

Keywords: Microfluidic Device High-throughput Dynamic Environmental Conditions Cell Culture Peristaltic Pump Shear-free Culture Stem Cell Differentiation Organ-on-chip

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