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Visualizing the Calcitonin Gene-Related Peptide Immunoreactive Innervation of the Rat Cranial Dura Mater with Immunofluorescence and Neural Tracing
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Capítulos
- 00:04Introduction
- 00:45Innervation of Rat Cranial Dura Mater
- 02:34Retrograde Tracing Study with Fluorogold
- 05:20Results: Neurovascular Structure of the Cranial Dura Mater and Retrograde-labeled Neurons in the TG and DRGs
- 07:02Conclusion
Here we present a protocol to visualize spatial correlation of calcitonin gene-related peptide (CGRP)-immunoreactive nerve fibers and blood vessels in the cranial dura mater using immunofluorescence and fluorescent histochemistry with CGRP and phalloidin, respectively. In addition, the origin of these nerve fibers was retrograde traced with a fluorescent neural tracer.
