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Evaluating the Angiogenetic Properties of Ovarian Cancer Stem-Like Cells using the Three-Dimensional Co-Culture System, NICO-1
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Biologia
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JoVE Journal Biologia
Evaluating the Angiogenetic Properties of Ovarian Cancer Stem-Like Cells using the Three-Dimensional Co-Culture System, NICO-1

Evaluating the Angiogenetic Properties of Ovarian Cancer Stem-Like Cells using the Three-Dimensional Co-Culture System, NICO-1

DOI:
10.3791/61751-v

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07:59 min

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December 05, 2020

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, , , , , , , , , , , , , ,
1Department of Obstetrics and Gynecology,Teikyo University School of Medicine, 2Department of Obstetrics and Gynecology,Niigata University Graduate School of Medical and Dental Sciences, 3Devision of Cancer Differentiation,National Cancer Center Research Institute

Capítulos

  • 00:05Introduction
  • 00:55Ovarian Cancer Stem Cell (OCSC) Isolation and Culture
  • 02:23CSC Passage
  • 03:24HUEhT-1 Endothelial Cell Culture
  • 04:14NICO-1 Coculture Plate Preparation
  • 05:16NICO-1 System HUEhT-1 and CSC Seeding
  • 06:14Results: Representative CSC-Induced Neovascularization and Vascular Formation
  • 07:19Conclusion

Summary

Tadução automática

Tadução automática

Ovarian cancer stem cells (OCSC) are responsible for cancer initiation, recurrence, therapeutic resistance, and metastasis. The OCSC vascular niche is considered to promote self-renewal of OCSCs, leading to chemoresistance. This protocol provides the basis for establishing a reproducible OCSC vascular niche model in vitro.

Tags

Ovarian CancerCancer Stem CellsAngiogenesis3D Co-culture SystemNICO-1Density Gradient CentrifugationOCSC MediumHUEhT-1 Endothelial CellsTumor MicroenvironmentParacentesisCell Detachment

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