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使用三维共培养系统NICO-1评估卵巢癌干细胞样细胞的血管遗传特性

JoVE Journal
Biologia

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JoVE Journal Biologia

Evaluating the Angiogenetic Properties of Ovarian Cancer Stem-Like Cells using the Three-Dimensional Co-Culture System, NICO-1

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使用三维共培养系统NICO-1评估卵巢癌干细胞样细胞的血管遗传特性

DOI:

10.3791/61751-v

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07:59 min

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December 05, 2020

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Yuko Miyagawa, Kazunori Nagasaka, Kaoru Yamawaki³, Yutaro Mori³, Tatsuya Ishiguro, Kei Hashimoto, Ryoko Koike, Siho Fukui, Takeru Sugihara, Takayuki Ichinose, Haruko Hiraike, Koichiro Kido, Koji Okamoto, Takayuki Enomoto, Takuya Ayabe

¹Department of Obstetrics and Gynecology,Teikyo University School of Medicine, ²Department of Obstetrics and Gynecology,Niigata University Graduate School of Medical and Dental Sciences, ³Devision of Cancer Differentiation,National Cancer Center Research Institute

Capítulos

00:05Introduction
00:55Ovarian Cancer Stem Cell (OCSC) Isolation and Culture
02:23CSC Passage
03:24HUEhT-1 Endothelial Cell Culture
04:14NICO-1 Coculture Plate Preparation
05:16NICO-1 System HUEhT-1 and CSC Seeding
06:14Results: Representative CSC-Induced Neovascularization and Vascular Formation
07:19Conclusion

Summary

Tadução automática

卵巢癌干细胞（OCSC）负责癌症的发生、复发、治疗耐药性和转移。OCSC血管生态位被认为可促进OCSC的自我更新，导致化学耐药性。该协议为体外建立可重复的OCSC血管生态位模型提供了基础。

Tags

Keywords: Ovarian Cancer Cancer Stem Cells Angiogenesis 3D Co-culture System NICO-1 Density Gradient Centrifugation OCSC Medium HUEhT-1 Endothelial Cells Tumor Microenvironment Paracentesis Cell Detachment

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