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Generering af humane neuroner og oligodendrocytter fra pluripotente stamceller til modellering af neuron-oligodendrocytinteraktioner
 
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Generering af humane neuroner og oligodendrocytter fra pluripotente stamceller til modellering af neuron-oligodendrocytinteraktioner

Article DOI: 10.3791/61778-v • 10:53 min • November 9th, 2020
November 9th, 2020 •
*1, *1,2, *3, 1, 1, 3, 3,4, 1,5,6
1Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, 2Department of Neurology, The Third Affiliated Hospital of Sun Yat-Sen University, 3Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, 4Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, 5Department of Neurology, Warren Alpert Medical School of Brown University, 6Center for Translational Neuroscience, Robert J. and Nancy D. Carney Institute for Brain Science and Brown Institute for Translational Science, Brown University
* These authors contributed equally

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Neuron-glial-interaktionerne i neurodegeneration forstås ikke godt på grund af utilstrækkelige værktøjer og metoder. Her beskriver vi optimerede protokoller til opnåelse af inducerede neuroner, oligodendrocytprecursorceller og oligodendrocytter fra humane pluripotente stamceller og giver eksempler på værdierne af disse metoder til forståelse af celletypespecifikke bidrag i Alzheimers sygdom.

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Neurovidenskab udgave 165 inducerede pluripotente stamceller iPS-celler humane embryonale stamceller ES-celler neuroner oligodendrocytter oligodendrocytprogenitorceller OPC'er Alzheimers sygdom amyloid-beta-peptider synapser
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