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使用卡诺哈布迪炎埃莱甘斯识别 EGFR 和 RAS 抑制剂

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Pesquisa do Câncer

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JoVE Journal Pesquisa do Câncer

Identification of EGFR and RAS Inhibitors using Caenorhabditis elegans

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使用卡诺哈布迪炎埃莱甘斯识别 EGFR 和 RAS 抑制剂

DOI:

10.3791/61788-v

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08:12 min

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October 05, 2020

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Dharini van der Hoeven², Thuy Nhu L. Truong, Ali Naji, Sabita Thapa, John F. Hancock, Ransome van der Hoeven

¹Department of Diagnostic and Biomedical Sciences, School of Dentistry,University of Texas Health Science Center, ²Department of Integrative Biology and Pharmacology, McGovern Medical School,University of Texas Health Science Center

Capítulos

00:05Introduction
00:26Preparation of a Synchronous C. elegans Culture
02:51Preparation of Drug Assays
04:30Observation of the Muv Phenotype in the let-60, let-23 and lin-1 Strains
06:38Results: R-fendiline Alters let-60 and let-23 Function in C. elegans in a Dose-dependent Manner
07:25Conclusion

Summary

Tadução automática

基因可处理的线虫 卡诺哈布迪炎的叶原体 可以用作药物发现一个简单和廉价的模型。这里描述的是一个协议，以确定抗癌治疗，抑制RAS和EGFR蛋白的下游信号。

Tags

EGFR RAS Caenorhabditis Elegans Drug Discovery Anti-EGFR Anti-RAS Therapeutics E. Coli OP50 NGM Plates Gravid Adult Worms Worm Lysis Embryos L1 Larvae M9W

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