/
/
Transillumination-assisterad dissekering av specifika stadier av mus seminiferous epitelial cykel för nedströms immunostaining analyser
This content is Free Access.
1Institute of Biomedicine, Integrative Physiology and Pharmacology Unit,University of Turku, Turku, Finland, 2Department of Pediatrics,Turku University Hospital, Turku, Finland, 3Centre for Reproductive Health,Hudson Institute of Medical Research, Melbourne, VIC 3168, Australia, 4Australian Regenerative Medicine Institute,Monash University, Melbourne, VIC 3800, Australia
Capítulos
- 00:04Introduction
- 00:51Testis Dissection and Preparation
- 02:10Transillumination-Assisted Microdissection
- 02:47Squash Preparation and Stage Verification
- 04:04Whole-Mount Seminiferous Tubule Immunostaining
- 07:04Results: Representative Mouse Seminiferous Epithelial Cycle Staging
- 09:16Conclusion
Detta protokoll beskriver transillumination-assisted microdissection av segment av vuxna mus seminiferous tubules representerar specifika stadier av seminiferous epitelial cykel och cell typer däri, och efterföljande immunostaining av squash preparat och intakt tubule segment.
