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マウス初代肝細胞単離のための時間と労力の効率的なプロトコルの改善
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Biology
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JoVE Journal
Biology
An Improved Time- and Labor- Efficient Protocol for Mouse Primary Hepatocyte Isolation
Please note that all translations are automatically generated.
Click here for the English version.
マウス初代肝細胞単離のための時間と労力の効率的なプロトコルの改善
DOI:
10.3791/61812-v
•
05:42 min
•
October 25, 2021
•
Mingxiao Feng
,
Sara Divall
,
Sheng Wu
2
1
Department of Pediatrics
,
Johns Hopkins University School of Medicine
,
2
Department of Physiology
,
Temple University School of Medicine
,
3
Department of Pediatrics
,
Seattle Children’s Hospital
Chapters
00:04
Introduction
00:45
Procedure
03:08
Results: Purity and Activity Levels of the Isolated Primary Hepatocytes
05:08
Conclusion
Summary
Automatic Translation
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Automatic Translation
初代肝細胞は、
インビトロで
肝臓応答および代謝を研究するための貴重なツールである。市販の試薬を利用して、マウス初代肝細胞単離のための改善された時間効率および労力効率の高いプロトコールが開発された。
Tags
Mouse Primary Hepatocytes
Liver Studies
Glucose Metabolism
Hepatic Drug Testing
Time-efficient
Labor-efficient
Protocol
Peristaltic Pump
Perfusion Medium
Collagenase Dispase Medium
Wash Medium
Cell Strainer
HBSS
Centrifugation
Cell Culture
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